FieldOngoing taxonomic work in the region will greatly facilitate specimen collection and identification. We expect to be able to collect and sample at least 250 species (including coralline algae) and 500 specimens from the current taxonomic list for Moorea, but that number could be higher. Voucher specimens of macroalgae will be processed and dried at Gump immediately after collecting, and deposited at the UPF Herbarium and UC Berkeley Herbaria. Photographs of algae in situ and after collection will be taken using a digital camera, and an image databank created to go alongside the field, morphological and collection data.
LaboratoryMicroscopic species will be sorted out and mounted under binocular and compound microscopes in the laboratory at Gump. Macroalgae requiring critical microscopic taxonomic identification using internal anatomy will be sampled and analyzed in the laboratory, and extensive use made of available algal taxonomic literature (N’Yeurt reprint collection andbooks), UPF herbarium specimens, and online databases (AlgaeBase). Microphotographs will be taken using a digital camera mounted on a microscope. Voucher slide specimens will be produced and deposited in UPF herbarium. Tissue samples will be provided for DNA extraction in Moorea and sequencing will be carried out at SI as for other Plants (see 1.1.6)
TimelineWe envision three field scientists for 75 days of collection, documentation and vouchering. A post-doctoral researcher (Dr. Antoine D. R. N’Yeurt) will be employed to process the samples for taxonomical identification and for targeted barcode sequences based on protocols established in collaboration with CBOL (Saunders, Alison, etc.).
Year 1: 75 days sampling effort; sample processing for both morphological and molecular identification; morphological identification
Year 2 - 3: Molecular work